Abbe Condenser:
A lens that is specially designed to mount under the stage and which
typically, moves in a vertical direction. An adjustable iris controls
the diameter of the beam of light entering the lens system. Both by
changing the size of this iris and by moving the lens toward or away
from the stage, the diameter and focal point of the cone of light that
goes through the specimen can be controlled. Abbe condensers are useful
at magnifications above 400X where the condenser lens has a numerical
aperture equal to or greater than the N.A. of the objective lens being
used.
Achromatic Lens:
A lens that helps to correct the misalignment of light that occurs when
it is refracted through a prism or lens. Since different color light
refracts at different angles, an achromatic lens is made of different
types of glass with varying indices of refraction. As a result, an
improved color alignment is achieved although not as good as is
achieved with plan or semi-plan objective lens. Most microscopes use
achromatic lens with more exacting applications requiring plan or
semi-plan objectives.
Arm: The part of the microscope that connects the eyepiece tube to the base.
Articulated Arm:
Part of a boom microscope stand, an articulated arm has one or more
joints to enable a greater variety of movement of the microscope head
and, as a result, more versatile range of viewing options.
Base: A microscope is typically composed of a head or body and a base. The base is the support mechanism.
Binocular Microscope:
A microscope with a head that has two eyepiece lens. Nowadays,
binocular is typically used to refer to compound or high power
microscopes where the two eyepieces view through a single objective
lens. A stereo (or low power microscope) may also have two eyepieces,
but since each eyepiece views through a separate objective lens, the
specimen appears in stereo (3-Dimensional). In order to distinguish
from monocular or trinocular microscopes, we have included both types
of binocular microscopes in our Binocular Microscope category.
Body:
Often referred to as the head, the body is the upper part of a
microscope including, eyepieces and objectives. Most modern microscopes
are modular in the sense that the same body can be used with different
bases and vice versa.
Boom Stand (Universal Boom Stand):
A microscope base that incorporates an adjustable arm or boom and
enables the body to be aligned in a variety of different positions.
Used in commercial inspection applications.
Calibration: The mathematical process of determining true distance when using a reticle.
Camera Adapter:
An adapter kit designed to enable a camera to fit on to the trinocular
port of a microscope (23mm or 30mm port diameter). The camera connects
to a step ring (or T-Mount) and then to the camera adapter.
CamAdapter: A trademark name for our CAS39 universal camera adapter kit sold only by The Microscope Store, LLC.
Clamp Base:
A clamp that replaces the traditional base on the bottom of a boom
microscope and enables the pole to be clamped on to the side of a work
bench or table.
C-Mount:
This is an adapter with a standard thread for mounting a lens to a
camera. It fits into a trinocular port. The mechanical standard is 1
diameter, 32 TPI (threads per inch), male on the lens and female on the
camera. The optical standard is that the image reaches the focal plane
at 17.5mm past the edge of the lens mounting threads.
Coarse Focus:
This is the knob on the side of the microscope that moves the objective
lens up and down. It is used in conjunction with the fine focus.
Coaxial Focus:
A focusing system with both the coarse and fine focusing knobs mounted
on the same axis. The coarse focus is typically the larger, outside
knob and vice versa. On some coaxial systems, the fine adjustment is
calibrated, allowing differential measurements to be recorded.
Comparison Microscope:
A microscope that enables side-by-side viewing of two different
specimens. The microscope has two sets of objectives with a single set
of eyepieces (monocular or binocular), often used in forensic science.
Compound Microscope:
Originally used to describe a microscope with more than one objective
lens, a compound microscope is now generally understood to be a high
power microscope with multiple, selectable objective lens of varied
magnifications. See stereo/low power.
Condenser:
A lens that concentrates the light on a specimen and increases the
resolution. Found in or below the stage on compound microscopes, only.
Contrast Plate: Found only on stereo microscopes, one side is white and one black. Either side can be used depending on your specimen.
Cover Slip:
A thin, square piece of glass or plastic placed over the specimen on a
microscope slide. It flattens out liquid samples and helps single plane
focusing.
Darkfield Microscopy:
a technique used to enhance the contrast in unstained specimens. It
works on the principle of illuminating the sample with light that will
not be collected by the objective lens, so not form part of the image.
This produces the classic appearance of a dark, almost black,
background with bright objects on it.
Darkfield Plate:
A circular iris that sits on the base of the microscope above the light
source and reflects the light horizontally to the specimen, thereby
achieving lateral illumination.
Digital Microscope: A microscope with a built in digital camera that enables direct feed to a PC, TV or printer.
DIN: Deutsches Institut für Normung, or in English, the German Institute for Standardization,
is an international standards organization that determines the
"standard" for a wide variety of different types of technology. "DIN
standard" microscope objective lenses use a 20mm diameter attachment
thread and are usually interchangeable between microscope manufacturers.
Dissecting Microscope: Typically interchangeable with stereo microscope, a dissecting microscope is a stereo microscope used in laboratory work.
Doublet Lens: A lens with two different lenses "welded" together. Used in widefield eyepieces to obtain improved color performance.
Dual-View:
A monocular microscope that has a second, vertical viewing port. Often
used by teachers. It can also be used for photographic applications.
Electron Microscope:
A type of microscope that uses electrons rather than light to create an
image of the target. It has much higher magnification or resolving
power than a normal light microscope, up to two million times, allowing
it to see smaller objects and details.
Eyepiece:
Otherwise referred to as an ocular, the eyepiece is the lens nearest to
your eye. Total magnification of a microscope is determined by the sum
of the eyepiece magnification multiplied by that of the objective lens.
Eyepiece Tube: The tube in which the eyepiece lens is situated.
Fine Focus: A knob used to fine tune the focus of a specimen in conjunction with the coarse focus.
Field of View: The diameter of the circle of light seen through a microscope.
Focus: The ability to achieve a clear image, typically achieved by moving either the eyepiece tubes or the stage.
Gem/Jeweler's Microscope:
A stereo microscope designed for viewing gems and jewelry, typically
incorporating an inclined pole, powerful zoom, darkfield plate and
intense, variable lighting.
Head: Often referred to as the body, it is the upper part of the microscope that includes the eyepiece tubes and prisms.
Illumination System: The light source on light microscopes, typically mounted under the stage except on inverted microscopes.
Immersion Oil:
A special oil used with the 100X objective in order to concentrate the
light and increase the resolution of the image. A drop of oil is placed
on the cover slip and the objective is lowered until it touches the
oil. There are two primary types of immersion oil: Type A and Type B;
Type B is more viscous.
Interpupillary Distance:
The distance between the two eyepieces, usually adjustable to fit
individual users. Inverted Microscope: A microscope designed with the
objectives under the stage and the light source above. Used for viewing
larger specimens, often in containers.
Iris Diaphragm:
Found on high power microscopes under the stage, the diaphragm is,
typically, a five hole-disc with each hole having a different diameter.
It is used to vary the light that passes through the stage opening and
helps to adjust both the contrast and resolution of a specimen. It is
particularly useful at higher powers.
Jeweler's Clip: A special clip that attaches to the stage and is designed to hold precious stones and jewelry for easier viewing.
Koehler Illumination:
A method of illumination named after August Koehler, the man who
invented it. It is also known as double diaphragm illumination because
it employs both a field and an aperture iris diaphragm to control the
illumination. If the light path is adjusted properly, it is possible to
enjoy the advantages of an evenly illuminated field, a bright image
without glare and minimum heating of the specimen.
Light Microscopes:
Any microscope that uses a source of light to create an image of the
specimen and, essentially includes all compound and stereo microscopes.
Magnification:
The essence of a microscope is its ability to magnify a specimen. Total
magnification of a microscope is determined by multiplying the
magnification capability of the eyepiece lens by that of the objective
lens.
Mechanical Stage:
A flat mechanism that sits on top of the stage and allows the viewer to
move a specimen small distances - a task that is otherwise difficult at
higher magnifications. Most mechanical stages are equipped with an X
and Y axis so the viewer can see how far the slide has moved.
Monocular Microscope: A compound microscope with a single eyepiece. Generally with two or three objectives.
Nosepiece: The upper part of a compound microscope that holds the objective lens. Also called a revolving nosepiece or turret.
Numerical Aperture (N.A):
A measure of the diameter of the aperture compared to the focal length
of a lens and ultimately, of the resolving power of a microscope. N.A.
is equal to the index of refraction of the medium in which the object
is placed multiplied by the sine of the angle made with the axis by the
most oblique ray entering the instrument, the resolving power
increasing as the product increases.
Objective Lens:
The lens closest to the specimen that first receives the rays from the
specimen (the object) and forms the image in the focal plane of the
eyepiece.
Oil Immersion Lens: Typically, a 100X (or higher) objective lens designed to work with a drop of immersion oil.
Parcentered: When changing objectives, the image of the specimen stays centered. Most compound microscopes are parcentered.
Parfocal:
When changing objectives, the image of the specimen stays in focus
without needing to adjust the focusing knobs. Most compound microscopes
are parfocal.
Phase Contrast:
A contrast enhancing technique developed by Frits Zernike in 1953 for
which he won the Nobel Prize in Physics. The technique shifts the light
phase wavelength, thereby causing the the light deviated by the
specimen to appear dark on a light background. It is useful for viewing
transparent specimens such as living tissue cells.
Plan Lens:
The finest objective lens that "flattens" the image of the specimen and
greatly enhances the resolution and clarity of the image.
Portable Microscope:
A cordless or field microscope with a light source independent of
110/220V. Typically, includes a rechargeable LED light source so that
it can be used in the field where 110/220V electric supply is
unavailable.
Pointer: A piece of high tensile wire that sits in the eyepiece and enables a viewer to point at a specific area of a specimen.
Pole Stand:
A microscope stand composed of a base with a single vertical pole (or
post). Typically, the body can move up and down as well as rotate round
the pole.
Rack and Pinion Focusing Mechanism: A metal rack and pinion used in better quality microscopes for focusing purposes and moving mechanical stages.
Rack Stop:
A safety feature that prevents the viewer from allowing the objective
lens to accidentally hit the stage and damage the specimen or slide.
Resolution: The ability of a lens to distinguish the fine details of the specimens being viewed.
Reticle:
A small glass circle, etched by laser with fine measurements and placed
within the eyepiece in order to enable actual measurements of the
specimen to be taken.
Revolving Nosepiece:
A nosepiece with multiple objectives that revolves in order to enable
the viewer to use, typically, one of four different objectives.
Ring Light:
An extraneous light source that connects to the microscope and emits a
ring of light for enhanced lighting. Ring lights are LED, fluorescent,
halogen or fiber optic and are typically, used on boom microscopes.
Seidentopf Head: A head design where the interpupillary adjustment is achieved by twisting the eyepieces in a vertical arc like binoculars.
Semi-Plan Objectives:
Improve the clarity and resolution of an image compared to chromatic
lens, by partially "flattening" the image of the specimen.
Slide: A flat, rectangular, glass plate on which a specimen may be placed.
Slip Clutch:
A mechanical device on the focusing knob that allows the knob to "slip"
if a viewer keeps turning the knob beyond its range of travel. Prevents
damage from occurring to the focusing system.
Stage: The platform on which slides and specimens are placed for viewing.
Stage Clips: Clips that are attached to the stage and retain the slide.
Stand: Describes the connection between the body and base a stereo or low power microscope
Stereo Microscope:
A low power microscope or dissecting microscope with a separate
eyepiece and objective lens for each eye. These separate optical
channels enable stereo or three-dimensional images of the specimen. See
Compound Microscope.
Sub-Stage: The parts of the microscope below the stage, including the illumination system.
T-Mount: A standard adapter for mounting 35mm cameras to microscopes. Also known as a step-ring.
Tension Adjustment:
A factory set adjustment to the focusing mechanism that ensures it is
both, sufficiently easy to focus and sufficiently tight to ensure that
the stage does not drift during the focusing process.
Turret: An alternative description for a nosepiece.
Widefield Eyepiece:
An improved eyepiece lens with a broader diameter that enables a
broader field of vision and greater ease of use.Some are equipped with
a pointer to identify specimens